Development of universal SYBR Green real-time RT-PCR for the rapid detection and quantitation of bovine and porcine toroviruses
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Development of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
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Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected cattle. qRT-PCR was optimized to quantify the number of BVD virus copies using Light Cycler® detection system and intercalation fluorogenic dye SYBR Green I. A universal set of primers was selected from a highly con...
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not available 687 Krakowka, S., Ringler, S.S., Arumugam, P. , McKillen, J., McIntosh, K., Hartunian, C., Hamberg, A., Rings, M., Allan, G., Ellis, J.A. (2008) Evaluation of Mycoplasma hyopneumoniae bacterins for porcine torque teno virus DNAs American Journal of Veterinary Research, 69, 1601-1607 Objective-TO determine whether commercial Mycoplasma hyopneumoniae bacterins sold for use in swine ...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2010
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2010.06.001